Drought has been shown to increase the activity of antioxidant enzymes such as catalase cat, ascorbate peroxidase apx and. Potential of antioxidant enzymes in depicting drought. A correlation analysis showed that antioxidant enzyme activities are inversely correlated to the expression levels of the ppatgs. Pdf antioxidant enzyme changes in response to drought. We hypothesized that the two shrubs had higher capacities of osmotic adjustment and antioxidant defense than the four trees, which permitted their higher drought tolerance and better photosynthetic performance under drought stress. Response of antioxidant enzymes to drought 399 figure 2. Role of abscisic acid and drought stress on the activities. The response of carbon metabolism and antioxidant defenses of. Antioxidant enzymes, wheat, seedling growth, drought, triticum aestivum l. Activity of photosynthetic enzymes the data on water stress induced regulation of the. Effect of drought stress on the antioxidant system and redox homeostasis. The response of carbon metabolism and antioxidant defenses.
Drought stress is considered as the main factor of yield limitations in arid and semiarid areas, where drought and salinity stresses are usually combined. Effects of drought stress on the seedling growth, development. Water stress is one of the severe limitations of crop growth especially in arid and semi. Oxidative stress in plants under drought conditions and. The activities of sod and pod were upregulated by salt and drought stress, while cat activity decreased. The effect of drought stress and recovery on the relative abundance of antioxidant transcripts.
Pdf effect of drought stress on antioxidant enzymes. Introduction based on various observations, water stress induces oxidative stress in plants manivannanet al. Ghanati 2 abstract drought is one of the most important abiotic stress es affecting plant growth and development. Response of antioxidant enzyme activities of sugar beet to. All these responses increase scavenging capacity against ros.
Accumulation of proline that synthesized from lglutamic acid with two enzymes of pyrroline5carboxylate p5c causes a mechanism that may store carbon and nitrogen. However, the responses of the antioxidant defense system in plant species to drought stress. Role of antioxidant enzymes in the ros scavenging mechanism. Morphological, physiochemical and antioxidant responses of. Role of abscisic acid and drought stress on the activities of. Pdf water stress, antioxidant enzyme activity and lipid. Comparison of these responses will help identify drought tolerance mechanisms in the melon cultivars k. Effects of drought stress on the lipid peroxidation and.
Effect of salt and drought stress on antioxidant enzymes. Impact of drought stress during germination on antioxidant. The role of the plant antioxidant system in drought tolerance. Another mechanism that could play a role in the droughtinduced inhibition of n 2 fixation, but has received much less attention, is oxidative stress. Effect of drought stress on antioxidant enzymes activity of.
Indeed, drought induces oxidative stress in pea nodules gogorcena et al. The activities of superoxide dismutase sod and peroxidase pod and contents of endogenous aba in plants were measured at 3 days of drought stress in cv. Cerasus humilis, grown in the northern areas of china, may experience water deficit during their life cycle, which induces oxidative stress. Plants cope with drought stress by manipulating key physiological processes like photosynthesis, respiration, water relations, antioxidant and hormonal metabolism. To meet the needs of the growing world population, it is essential to effectively utilize dehydrated soil in droughtprone areas. Effect of salicylic acid application on oxidative damage and. The scavenging system in drought tolerant variety introduction drought is one of the major abiotic stresses. Dec 17, 2019 drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in fraxinus ornus leaves. Antioxidant enzymes activities pox, ppo, sod, cat and apx were significantly increased accompanied by significant decreases in nr due to drought stress. The most effective level of drought stress to elevate the capabilities and enzyme activity of antioxidant as well as it gene expression was observed at 15% peg, whereas abts scavenging activity was increased 20% over the control, hydroxyl scavenging activity up to 14%, cat and apx activity increased 4 5 times over the control.
Amounts of malondialdehyde mda and hydrogen peroxide h2o2 remained unchanged in sesupplemented waterdeficit plants obviously because of an efficient scavenging following significant enhancement of catalase cat and glutathione peroxidase gshpx activities. Additionally the activity of antioxidant enzymes sod, apx, dhar and gr resulted affected by drought stress. Pdf effects of drought stress on antioxidant enzymes in. To evaluate the effects of drought stress on antioxidant enzymes activity of three chickpea cultivars, an experiment was conducted at the experimental field of the islamic azad university, karaj branch and laboratories of tarbiyat moallem university of tehran. Antioxidant enzymes, drought stress, grapevine, malondialdehyde, salicylic acid. Effect of potassium levels on antioxidant enzymes and. Drought stressinduced oxidative stress and antioxidative responses in four wheat triticum aestivum l. For field studies, a spiltsplit plot design on the basis of rcbd with four replications was used. Abiotic stresses, such as drought, soil salinity and extreme temperatures adversely affect the productivity and quality of economically important. The effect of various polyethylene glycol peg concentrations to induced drought stress on the antioxidant capacities and antioxidant enzyme activities was investigated on madura local maize during germination. Microbial inoculation in rice regulates antioxidative. Pdf in arid and semi arid zones, soil moisture deficit as a major adverse factor can lower leaf water potential, leading to reduced turgor and some.
Antioxidant enzyme responses and crop yield of wheat under. Drought effects are a high risk to rice production, especially in parts of peninsular malaysia. The role of the plant antioxidant system in drought tolerance mdpi. The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape brassica napus l. Enhancement of antioxidant defense in plants can thus increase tolerance to different stress factors. Influence of drought stress on assimilation of carbon, activity of photosynthetic enzymes, heat shock proteins, antioxidants. Physiological role of glycinebetaine in alleviating the. Drought tolerance is correlated with the activity of. Reactive oxygen species and antioxidant enzymes involved. Effect of soil drought stress on leaf water status, membrane. Steadystate levels of mrnas encoding antioxidant enzymes in the bacteroids of alfalfa plants exposed to drought stress and following recovery from drought. Drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in fraxinus ornus leaves. These multifarious modulations in plants were related to stress.
Plant treatments are designated as in figure 2 and statistical analysis is as described in figure 4. Microbial inoculation in drought challenged rice triggered multipronged steps at enzymatic, nonenzymatic and gene expression level. Drought stress disturbs water relations, causing a reduction in wateruse efficiency. During drought stress, upregulation of antioxidant systems occurs at both the transcriptional and posttranscriptional level. Although, this crop is adapted to resist severe drought 17, little is known about its mechanisms of osmotic adjustment and ability to repair the damage caused by droughtinduced oxidative stress. Under drought stress, the photosynthetic abilities of peach leaves decreased, while antioxidant enzyme activities, autophagy and atg expression increased. Seed samples were treated under 5%, 10%, and 15% of peg for 6 days. Oxidative stress in five wheat varieties triticum aestivum l. Effect of soil drought stress on leaf water status. It is the major yieldlimiting factor of crop plants and it actively and continuously determines the natural distribution of plant species. In particular, it has been investigated for its roles in abiotic stress management. Some antioxidant enzymes such as sod, cat, apx, and gpx are better studied figure 3 and 4, but in general all enzymes have potential defense antioxidant activity helping in scavenging ros in different ways, either by dismutation of o 2 to h 2 o 2, reduction of h 2 o 2, maintenance of the asa pool, or of the adequate levels of gsh and gssg.
Read effect of salt and drought stress on antioxidant enzymes activities and sod isoenzymes of liquorice glycyrrhiza uralensis fisch, plant growth regulation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. Further, very little is known, about the link between drought stress, lox activity, antioxidant enzymes, pro and mda levels, response in the millets. Effects of coronatine on growth, gas exchange traits. When rice seedlings grown for 10 and 20 days were subjected to in vitro drought stress of. Effect of salicylic acid application on oxidative damage. Exogenous melatonin confers drought stress by promoting. American journal of agricultural and biological sciences. Effect of drought stress on oxidative damage and antioxidant. The effects of salinity and drought on the antioxidative system sod, pod, cat were studied in liquorice seedlings glycyrrhiza uralensis fisch. Although, this crop is adapted to resist severe drought 17, little is known about its mechanisms of osmotic adjustment and ability to repair the damage caused by drought induced oxidative stress. The effect of drought stress and abscisic acid aba applied at tillering stage 55 days after sowing was compared in 2 wheat cultivars differing in drought tolerance.
Drought has been shown to increase the activity of antioxidant enzymes such as catalase cat, ascorbate peroxidase apx and guaiacol peroxidase gpx 7,10,11. Article pdf available april 2020 with 70 reads how we measure reads. Oxidative stress in plants under drought conditions and the role of different enzymes leonora mansur mattos and celso luiz moretti brazilian agricultural research corporation, brazil e n z y m e e ngi n e e r i n issn. Effect of potassium levels on antioxidant enzymes and malondialdehyde content under drought stress in sunflower helianthus annuus l.
However, studies on biochemical factors and antioxidant enzymes during drought stress in a drought tolerant crop like horsegram still remain elusive. Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola brassica napus l. Drought stress preferentially enhanced the activities of superoxide dismutase sod and guaiacol peroxidase pod whereas it decreased catalase cat. In plant cells, this occurs when the generation of reactive oxygen species ros overwhelms the antioxidant defenses. The current experiment was carried out to investigate the protective role of melatonin in photosynthetic traits and the antioxidant defense system of maize seedling under drought stress. Drought stressinduced oxidative stress and antioxidative. Melatonin is an important biologically active hormone that plays a vital role in plant growth and development. Influence of drought stress on assimilation of carbon.
Nonavailability of highyielding varieties suitable for cultivation under drought condition lead towards a sharp decline in rice yield. Exogenous melatonin confers drought stress by promoting plant growth, photosynthetic capacity and antioxidant defense system of maize seedlings shakeel ahmad 1, 2, muhammad kamran 1, 2, 3, ruixia ding 1, 2, xiangping meng 1, 2, haiqi wang 1, 2, irshad ahmad 1, 2, shah fahad 4, qingfang han 1, 2. Effect of drought stress on growth parameters, osmolyte. The effect of micronutrients on antioxidant enzymes.
Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. To evaluate the effects of drought stress on antioxidant enzymes activity of three chickpea cultivars, an experiment was conducted at the experimental field of the islamic azad university, karaj branch and laboratories of tarbiyat moallem. Growth performance and antioxidant enzyme activities of. Drought stress increased mean germination rate and mean of day germination in both cultivars, and its effect was more pronounced in rgs003. The results of biochemical analysis showed the activity of antioxidant enzymes increases under stress conditions. The objective of this study was to investigate the effect of micronutrients application on the antioxidant enzymes metabolism of sunflower oil under drought stress.
Regulation of pea cytosolic ascorbate peroxidase and other. Exogenous melatonin confers drought stress by promoting plant. The effects of slight, moderate and intense drought 70, 35 and 23% of the regular irrigation, respectively on growth parameters, osmolyte content, oxidative stress markers, antioxidant enzymes, glycyrrhizin biosynthesis genes and root glycyrrhizin concentration and contents, have been assessed in five iranian licorice genotypes grown in the. Antioxidant enzymes regulated in plants under drought. Effects of drought stress on antioxidant enzymes in seedlings of different wheat genotypes article pdf available in pakistan journal of botany 471. Drought stress, potassium fertilizer, antioxidant enzymes, sunflower introduction adequate water and nutrient supply are important factors affecting optimal plant growth and successful crop production. Stage specific upregulation of antioxidant defence system in. Effects of coronatine on growth, gas exchange traits, chlorophyll content, antioxidant enzymes and lipid peroxidation in maize zea mays l. There exist multiple and often redundant stress sensors, which transduce the stress signal through secondary signalling. Drought is one of the most important manifestations of abiotic stress in plants. As past research has shown, environmental drought stress has caused oxidative damage in plants larson, 1995. The results of this study suggested that the amount of antioxidant enzymes determine drought stress tolerance in wheat. Drought induces oxidative stress and enhances the activities. Effect of drought stress on antioxidant enzymes activity.
Effect of drought on pigments, osmotic adjustment and. The effect of drought stress and recovery from drought on the relative abundance of. Antioxidant enzyme changes in response to drought stress in ten. The antioxidant system including antioxidant enzymes such as superoxide dismutase sod, peroxidase pod, and catalase cat as. Application of 20 gha selenium increased enzymes activity except the catalase. Drought stress affects various physiological and metabolic processes in rice oryza sativa l. To overcome oxidative damage under drought stress conditions, plants must have efficient antioxidant system stepien and klobus, 2005. Abstractthe comparative effects of drought stress on seed performance, growth parameters, free proline content, lipid peroxidation, and several antioxidative enzymes activities were studied in both cultivars of brassica napus l. Impact of seed priming on proline content and antioxidant. Leaf is one of the early sensors for the drought stress and is important to study drought tolerance mechanism. Drought tolerance is correlated with the activity of antioxidant. Exposure to abiotic stresses including drought, chilling, salinity, etc.
Reactive oxygen species and antioxidant enzymes involved in. Induce mutation is an essential auxiliary approach to counterpart conventional breeding to produce stress tolerance rice variety. The changes in the antioxidant capacities such as 2,2azinobis3ethylbenzthiazoline6sulphonic acid abts and. High activities of antioxidant enzymes were also shown to improve drought tolerance of genotypes of tea upadhyaya et al. Various related research indicates that the capacities and activities of antioxidant enzymes are correlated with plant resistance to abiotic stress, including drought stress 5 8 12 14. Drought is perceived as the most significant environmental stress in agriculture worldwide, and improving yield under drought is therefore a major goal of plant breeding. The antioxidant defence system in the plant cell comprises both enzymatic and nonenzymatic components. The results showed that both salt and drought stresses could induce oxidative stress, as indicated by the increase level of lipid peroxidation. Drought stress at 75% fc and 50% fc decreased seed yieldplant, oil and carbohydrate, total phenolic content, tannins, flavonoids and antioxidant activity accompanied by significant. Singh division of biochemistry, iari, new delhi110012, india corresponding author a b s t r a c t peroxidase enzymatic activities measured.
Sep 29, 2006 the effects of salinity and drought on the antioxidative system sod, pod, cat were studied in liquorice seedlings glycyrrhiza uralensis fisch. Oxidative stress in plants under drought conditions and the. Kochia showed high tolerance against drought and salinity stresses and different antioxidant enzymes had different behavior under stress conditions. Material and method this experiment was carried out at the golmakan agriculture research station iran in. Pdf effect of drought stress on water status, elecrolyte. Antioxidant enzymes to mitigate drought stress in rice genotype m. Table 4 gives examples for quantitative drought responses of antioxidative enzymes and enzymes involved in regeneration of nonprotein.
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