Growth performance and antioxidant enzyme activities of. Water stress is one of the severe limitations of crop growth especially in arid and semi. Effect of potassium levels on antioxidant enzymes and malondialdehyde content under drought stress in sunflower helianthus annuus l. The antioxidant defence system in the plant cell comprises both enzymatic and nonenzymatic components. Effect of potassium levels on antioxidant enzymes and. Activity of photosynthetic enzymes the data on water stress induced regulation of the.
Drought stress preferentially enhanced the activities of superoxide dismutase sod and guaiacol peroxidase pod whereas it decreased catalase cat. The effect of drought stress and recovery from drought on the relative abundance of. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. Exposure to abiotic stresses including drought, chilling, salinity, etc.
However, studies on biochemical factors and antioxidant enzymes during drought stress in a drought tolerant crop like horsegram still remain elusive. Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola brassica napus l. Drought stress affects various physiological and metabolic processes in rice oryza sativa l. Finally, this research indicated that the bivanij cultivar is the most tolerant to drought stress among the studied cultivars. Effect of salicylic acid application on oxidative damage and. It is the major yieldlimiting factor of crop plants and it actively and continuously determines the natural distribution of plant species.
Drought has been shown to increase the activity of antioxidant enzymes such as catalase cat, ascorbate peroxidase apx and guaiacol peroxidase gpx 7,10,11. A correlation analysis showed that antioxidant enzyme activities are inversely correlated to the expression levels of the ppatgs. Effect of drought stress on growth parameters, osmolyte. Singh division of biochemistry, iari, new delhi110012, india corresponding author a b s t r a c t peroxidase enzymatic activities measured. The effect of drought stress and recovery on the relative abundance of antioxidant transcripts. The scavenging system in drought tolerant variety introduction drought is one of the major abiotic stresses. The response of carbon metabolism and antioxidant defenses. Antioxidant enzymes activities pox, ppo, sod, cat and apx were significantly increased accompanied by significant decreases in nr due to drought stress. The activities of superoxide dismutase sod and peroxidase pod and contents of endogenous aba in plants were measured at 3 days of drought stress in cv.
To evaluate the effects of drought stress on antioxidant enzymes activity of three chickpea cultivars, an experiment was conducted at the experimental field of the islamic azad university, karaj branch and laboratories of tarbiyat moallem university of tehran. Effect of drought stress on the antioxidant system and redox homeostasis. In plant cells, this occurs when the generation of reactive oxygen species ros overwhelms the antioxidant defenses. To overcome oxidative damage under drought stress conditions, plants must have efficient antioxidant system stepien and klobus, 2005. Under drought stress, the photosynthetic abilities of peach leaves decreased, while antioxidant enzyme activities, autophagy and atg expression increased.
These multifarious modulations in plants were related to stress. Further, very little is known, about the link between drought stress, lox activity, antioxidant enzymes, pro and mda levels, response in the millets. When rice seedlings grown for 10 and 20 days were subjected to in vitro drought stress of. Table 4 gives examples for quantitative drought responses of antioxidative enzymes and enzymes involved in regeneration of nonprotein. To meet the needs of the growing world population, it is essential to effectively utilize dehydrated soil in droughtprone areas. Drought is perceived as the most significant environmental stress in agriculture worldwide, and improving yield under drought is therefore a major goal of plant breeding. The response of carbon metabolism and antioxidant defenses of. Reactive oxygen species and antioxidant enzymes involved. Impact of drought stress during germination on antioxidant. For field studies, a spiltsplit plot design on the basis of rcbd with four replications was used. Article pdf available april 2020 with 70 reads how we measure reads. The results of this study suggested that the amount of antioxidant enzymes determine drought stress tolerance in wheat. All these responses increase scavenging capacity against ros. Read effect of salt and drought stress on antioxidant enzymes activities and sod isoenzymes of liquorice glycyrrhiza uralensis fisch, plant growth regulation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at.
The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape brassica napus l. The results showed that both salt and drought stresses could induce oxidative stress, as indicated by the increase level of lipid peroxidation. The effects of slight, moderate and intense drought 70, 35 and 23% of the regular irrigation, respectively on growth parameters, osmolyte content, oxidative stress markers, antioxidant enzymes, glycyrrhizin biosynthesis genes and root glycyrrhizin concentration and contents, have been assessed in five iranian licorice genotypes grown in the. Drought stress is one of the most important abiotic stress factors which are generally accompanied by heat stress in dry season. Influence of drought stress on assimilation of carbon, activity of photosynthetic enzymes, heat shock proteins, antioxidants. Drought stressinduced oxidative stress and antioxidative responses in four wheat triticum aestivum l. Oxidative stress in five wheat varieties triticum aestivum l. The effects of salinity and drought on the antioxidative system sod, pod, cat were studied in liquorice seedlings glycyrrhiza uralensis fisch. However, the responses of the antioxidant defense system in plant species to drought stress. Although, this crop is adapted to resist severe drought 17, little is known about its mechanisms of osmotic adjustment and ability to repair the damage caused by droughtinduced oxidative stress. Antioxidant enzymes, wheat, seedling growth, drought, triticum aestivum l. Leaf is one of the early sensors for the drought stress and is important to study drought tolerance mechanism. Microbial inoculation in drought challenged rice triggered multipronged steps at enzymatic, nonenzymatic and gene expression level.
During drought stress, upregulation of antioxidant systems occurs at both the transcriptional and posttranscriptional level. Pdf effect of drought stress on water status, elecrolyte. The effect of micronutrients on antioxidant enzymes. Stage specific upregulation of antioxidant defence system in. Accumulation of proline that synthesized from lglutamic acid with two enzymes of pyrroline5carboxylate p5c causes a mechanism that may store carbon and nitrogen. Effect of soil drought stress on leaf water status, membrane. Drought tolerance is correlated with the activity of. To evaluate the effects of drought stress on antioxidant enzymes activity of three chickpea cultivars, an experiment was conducted at the experimental field of the islamic azad university, karaj branch and laboratories of tarbiyat moallem.
Oxidative stress in plants under drought conditions and the role of different enzymes leonora mansur mattos and celso luiz moretti brazilian agricultural research corporation, brazil e n z y m e e ngi n e e r i n issn. Ghanati 2 abstract drought is one of the most important abiotic stress es affecting plant growth and development. Effect of drought on pigments, osmotic adjustment and. Drought tolerance is correlated with the activity of antioxidant. Potential of antioxidant enzymes in depicting drought. Physiological role of glycinebetaine in alleviating the. The current experiment was carried out to investigate the protective role of melatonin in photosynthetic traits and the antioxidant defense system of maize seedling under drought stress. Response of antioxidant enzyme activities of sugar beet to. Drought effects are a high risk to rice production, especially in parts of peninsular malaysia. Response of antioxidant enzymes to drought 399 figure 2. Exogenous melatonin confers drought stress by promoting plant growth, photosynthetic capacity and antioxidant defense system of maize seedlings shakeel ahmad 1, 2, muhammad kamran 1, 2, 3, ruixia ding 1, 2, xiangping meng 1, 2, haiqi wang 1, 2, irshad ahmad 1, 2, shah fahad 4, qingfang han 1, 2.
Nonavailability of highyielding varieties suitable for cultivation under drought condition lead towards a sharp decline in rice yield. Various related research indicates that the capacities and activities of antioxidant enzymes are correlated with plant resistance to abiotic stress, including drought stress 5 8 12 14. Antioxidant enzyme changes in response to drought stress in ten. As past research has shown, environmental drought stress has caused oxidative damage in plants larson, 1995. Effects of drought stress on the seedling growth, development. High activities of antioxidant enzymes were also shown to improve drought tolerance of genotypes of tea upadhyaya et al. Dec 17, 2019 drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in fraxinus ornus leaves. Exogenous melatonin confers drought stress by promoting plant. Oxidative stress in plants under drought conditions and the. Effect of drought stress on antioxidant enzymes activity of. We hypothesized that the two shrubs had higher capacities of osmotic adjustment and antioxidant defense than the four trees, which permitted their higher drought tolerance and better photosynthetic performance under drought stress. Drought is one of the most important manifestations of abiotic stress in plants. The activities of sod and pod were upregulated by salt and drought stress, while cat activity decreased. Another mechanism that could play a role in the droughtinduced inhibition of n 2 fixation, but has received much less attention, is oxidative stress.
Regulation of pea cytosolic ascorbate peroxidase and other. Application of 20 gha selenium increased enzymes activity except the catalase. Reactive oxygen species and antioxidant enzymes involved in. Material and method this experiment was carried out at the golmakan agriculture research station iran in. Cerasus humilis, grown in the northern areas of china, may experience water deficit during their life cycle, which induces oxidative stress. Seed samples were treated under 5%, 10%, and 15% of peg for 6 days. Effects of drought stress on the lipid peroxidation and. Pdf antioxidant enzyme changes in response to drought. Abstractthe comparative effects of drought stress on seed performance, growth parameters, free proline content, lipid peroxidation, and several antioxidative enzymes activities were studied in both cultivars of brassica napus l.
Plants cope with drought stress by manipulating key physiological processes like photosynthesis, respiration, water relations, antioxidant and hormonal metabolism. Effects of drought stress on antioxidant enzymes in seedlings of different wheat genotypes article pdf available in pakistan journal of botany 471. Antioxidant enzymes regulated in plants under drought. The effect of drought stress and abscisic acid aba applied at tillering stage 55 days after sowing was compared in 2 wheat cultivars differing in drought tolerance. Indeed, drought induces oxidative stress in pea nodules gogorcena et al. American journal of agricultural and biological sciences. Antioxidant enzymes, drought stress, grapevine, malondialdehyde, salicylic acid. There exist multiple and often redundant stress sensors, which transduce the stress signal through secondary signalling. Additionally the activity of antioxidant enzymes sod, apx, dhar and gr resulted affected by drought stress. Role of antioxidant enzymes in the ros scavenging mechanism. Effect of soil drought stress on leaf water status. Effect of drought stress on antioxidant enzymes activity. Abiotic stresses, such as drought, soil salinity and extreme temperatures adversely affect the productivity and quality of economically important. Drought stress, potassium fertilizer, antioxidant enzymes, sunflower introduction adequate water and nutrient supply are important factors affecting optimal plant growth and successful crop production.
Drought stress increased mean germination rate and mean of day germination in both cultivars, and its effect was more pronounced in rgs003. The results of biochemical analysis showed the activity of antioxidant enzymes increases under stress conditions. The changes in the antioxidant capacities such as 2,2azinobis3ethylbenzthiazoline6sulphonic acid abts and. In particular, it has been investigated for its roles in abiotic stress management. Drought stressinduced oxidative stress and antioxidative. Pdf in arid and semi arid zones, soil moisture deficit as a major adverse factor can lower leaf water potential, leading to reduced turgor and some. Role of abscisic acid and drought stress on the activities of. Pdf effect of drought stress on antioxidant enzymes. Influence of drought stress on assimilation of carbon. The role of the plant antioxidant system in drought tolerance. Oxidative stress in plants under drought conditions and.
Drought induces oxidative stress and enhances the activities. Effect of drought stress on oxidative damage and antioxidant. The most effective level of drought stress to elevate the capabilities and enzyme activity of antioxidant as well as it gene expression was observed at 15% peg, whereas abts scavenging activity was increased 20% over the control, hydroxyl scavenging activity up to 14%, cat and apx activity increased 4 5 times over the control. Kochia showed high tolerance against drought and salinity stresses and different antioxidant enzymes had different behavior under stress conditions. Amounts of malondialdehyde mda and hydrogen peroxide h2o2 remained unchanged in sesupplemented waterdeficit plants obviously because of an efficient scavenging following significant enhancement of catalase cat and glutathione peroxidase gshpx activities. Sep 29, 2006 the effects of salinity and drought on the antioxidative system sod, pod, cat were studied in liquorice seedlings glycyrrhiza uralensis fisch. Exogenous melatonin confers drought stress by promoting. Drought stress is considered as the main factor of yield limitations in arid and semiarid areas, where drought and salinity stresses are usually combined. Effects of coronatine on growth, gas exchange traits, chlorophyll content, antioxidant enzymes and lipid peroxidation in maize zea mays l. Introduction based on various observations, water stress induces oxidative stress in plants manivannanet al.
Effects of coronatine on growth, gas exchange traits. Effect of salicylic acid application on oxidative damage. Comparison of these responses will help identify drought tolerance mechanisms in the melon cultivars k. Microbial inoculation in rice regulates antioxidative.
Role of abscisic acid and drought stress on the activities. Induce mutation is an essential auxiliary approach to counterpart conventional breeding to produce stress tolerance rice variety. The antioxidant system including antioxidant enzymes such as superoxide dismutase sod, peroxidase pod, and catalase cat as. Melatonin is an important biologically active hormone that plays a vital role in plant growth and development. Pdf effects of drought stress on antioxidant enzymes in.
Plant treatments are designated as in figure 2 and statistical analysis is as described in figure 4. Drought has been shown to increase the activity of antioxidant enzymes such as catalase cat, ascorbate peroxidase apx and. Although, this crop is adapted to resist severe drought 17, little is known about its mechanisms of osmotic adjustment and ability to repair the damage caused by drought induced oxidative stress. Steadystate levels of mrnas encoding antioxidant enzymes in the bacteroids of alfalfa plants exposed to drought stress and following recovery from drought.
Drought stress disturbs water relations, causing a reduction in wateruse efficiency. Enhancement of antioxidant defense in plants can thus increase tolerance to different stress factors. Morphological, physiochemical and antioxidant responses of. Some antioxidant enzymes such as sod, cat, apx, and gpx are better studied figure 3 and 4, but in general all enzymes have potential defense antioxidant activity helping in scavenging ros in different ways, either by dismutation of o 2 to h 2 o 2, reduction of h 2 o 2, maintenance of the asa pool, or of the adequate levels of gsh and gssg.
Antioxidant enzyme responses and crop yield of wheat under. Impact of seed priming on proline content and antioxidant. The effect of various polyethylene glycol peg concentrations to induced drought stress on the antioxidant capacities and antioxidant enzyme activities was investigated on madura local maize during germination. Antioxidant enzymes to mitigate drought stress in rice genotype m. Effect of salt and drought stress on antioxidant enzymes. The role of the plant antioxidant system in drought tolerance mdpi. Drought stress at 75% fc and 50% fc decreased seed yieldplant, oil and carbohydrate, total phenolic content, tannins, flavonoids and antioxidant activity accompanied by significant. Therefore, the present study was framed to compare the biochemical and antioxidative responses in leaf and root tissue of a drought sensitive and a drought tolerant variety of horsegram under. Pdf water stress, antioxidant enzyme activity and lipid.
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